A CRISPR/dCas9 toolkit for functional analysis of maize genes
Abstract Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants.The RNA-guided nuclease can be used to pedal left not only generate precise genomic mutations, but also to manipulate gene expression when present as a deactivated protein (dCas9).Result